Lab Specialist Intermediate or Senior , Department of Surgery

The Brayman Lab in the Department of Surgery at the University of Virginia is seeking a Laboratory Specialist Intermediate or Senior to perform and assist with studies investigating the underlying mechanisms of type 1 diabetes and recurrence of diabetes in various murine models of diabetes. The position will also support human pancreatic islet isolations in the GMP islet isolation facility.

Responsibilities

Research responsibilities include working with mice and performing/assisting with in vitro and in vivo experiments related to ongoing studies, including: Working with murine models of diabetes, including mouse islet isolation and transplantation Surgical procedures Isolating islets Measuring mouse blood glucose levels Collection of mouse tissue, serum, and blood, including collection of mouse tissue and blood for proteomic studies Assistance with collection and entry of data in electronic media Supporting daily lab activities, maintenance, and general laboratory upkeep Ordering/purchasing materials and supplies, including purchasing through the procurement system Other duties as assigned The ideal candidate is an enthusiastic team-oriented individual with a willingness to learn, collaborate, and take on new challenges. Candidates must be able to: Perform routine laboratory data analysis and research procedures Independently prepare solutions and reagents Perform or learn to work with animal models (including surgical techniques; training provided if necessary) Work diligently with strong attention to detail Analyze and organize work procedures; plan and execute tasks/experiments; interpret and report findings, writing skills Work a flexible schedule when required Train in and comply with BSL-2 procedures Demonstrate reproducibility and strong critical thinking skills

Minimum Qualifications

Education: Bachelor's degree required.

Experience

3+ years of relevant experience.

Preferred Qualifications

Experience working actively in a research laboratory; experience in standard cell biology techniques and murine work Flow cytometry and CyTOF Protein purification (including immunoglobulins such as IgM) and chromatography Experience with Akta go FPLC system Proteomics Experience with mass spectroscopy (LC-MS), glycan preparation, data analysis Immunoassays, including ELISAs Electrophoresis and Western blot PCR, RT-PCR DNA and RNA extractions for NGS, RNAseq/ scRNAseq/ cfRNAseq Tissue culture / in vitro cell culture Histology analysis and immunostaining of tissue sections to back up imaging mass cytometry Immunohistochemistry, Immunofluorescence, Immunocryohistochemistry. Stem cells and organoids Imaging mass cytometry Confocal Experience in R and Python, with additional experience in downstream pipeline analysis and additional expertise in statistical and data analysis software Physical Demands This position can spend extensive periods of time standing and squatting while conducting experiments or caring for animals. Also requires keyboarding and other bench work and animal procedures requiring finer finger manipulation.